Researchers from North-West University (NWU) have uncovered a critical concern in South Africa’s water systems. In their report, Quantification, Fate, and Hazard Assessment of HIV-ARVs in Water Resources, they revealed significant concentrations of antiretroviral drugs (ARVs) in water sources, particularly downstream of wastewater treatment plants. This study underscores the environmental and health risks posed by these pharmaceuticals, driven by South Africa’s extensive HIV treatment programme, the largest globally.

Professors Suranie Horn, Rialet Pieters, Henk Bouwman, and Petra Bester led this pivotal research. The most frequently detected ARVs, lopinavir and efavirenz, were found at concentrations exceeding international standards at several sites.
“Although not found in this study, humans and other organisms may potentially ingest antiretrovirals and their breakdown products via aquatic organisms such as fish,” the study read, highlighting concerns about indirect exposure through the food chain.
The study revealed significant impacts on aquatic ecosystems and wastewater management systems. Freshwater snails exposed to ARVs exhibited altered embryonic development, while bacteriophages—viruses critical for controlling bacteria in wastewater treatment—suffered severe disruption.
“Such disruptions could lead to bacterial blooms and reduced water quality,” the study read. Additionally, it noted that humans are exposed to ARVs through drinking water at concentrations exceeding calculated hazard quotients, posing potential long-term health risks.
The Department of Water and Sanitation (DWS), through Spokesperson Wisane Mavasa, acknowledged these findings, noting that municipal wastewater treatment systems are not designed to remove pharmaceutical compounds, allowing ARVs to contaminate water sources.
Furthermore, the study advocates establishing regulatory criteria for ARV concentrations, upgrading wastewater treatment technologies, and conducting further research to assess ecological and human health impacts.
Therefore, these findings highlight the urgent need for communities to test their tap water for contamination.
The DWS’s 2023 Blue Drop Audit Report revealed that 46% of water supply systems are microbiologically unsafe, posing acute health risks due to bacteria such as Escherichia coli. The Green Drop Report further indicated that 67.6% of wastewater treatment works are nearing failure, contributing to widespread pollution of rivers and tap water. The Department of Cooperative Governance and Traditional Affairs (CoGTA) noted that ageing infrastructure and inadequate maintenance exacerbate these issues, particularly in rural municipalities, where only 64.2% of households in Limpopo and 67.2% in the Eastern Cape have access to piped water, according to their 2023 municipal service delivery assessments CoGTA.
The South African Human Rights Commission (SAHRC) has declared water contamination a human rights crisis, stating in its 2023 Water and Sanitation Report that nearly 50% of municipalities provide water failing to meet the South African National Standard (SANS 241:2015) for drinking water quality SAHRC. This is particularly severe in informal settlements, where poor sanitation infrastructure allows untreated waste to contaminate water sources through runoff.
Additionally, civil rights organisations, such as the Centre for Environmental Rights (CER), stress that inadequate enforcement of the Water Services Act of 1997 contributes to systemic failures, with many municipalities lacking the technical capacity to maintain treatment facilities.
Lastly, the Water Research Commission (WRC) has identified pharmaceutical pollution, including ARVs, as an emerging concern, driven by South Africa’s 7.9 million HIV-positive population, with over 5 million on ARV treatment. Research into remediation technologies, such as algae-based treatment, is ongoing but not yet widely implemented.
These challenges underscore the critical need for communities to proactively test their tap water and advocate for improved water management. The DWS, through its complaints portal DWS, and the SAHRC, via [email protected], provide avenues for reporting water quality issues. Regular monitoring and community engagement are essential to uphold the constitutional right to safe water, as enshrined in Section 27 of the South African Constitution.
Given the dire state of South Africa’s water supply, Newcastillian News provides South African residents with practical steps to test their tap water for contamination, addressing microbiological, chemical (including ARVs), and physical contaminants.
This guide is informed by official sources such as the Department of Water and Sanitation (DWS), the South African Human Rights Commission (SAHRC), and verified institutions like the Water Institute of Southern Africa (WISA) and the Council for Scientific and Industrial Research (CSIR).
Step 1: Know What You’re Testing For
Before you dive into testing your tap water, it helps to understand what you’re actually checking for. There are three main types of contaminants:
- Germs (Microbiological Contaminants): These include bacteria like E. coli and coliforms. If found, it usually means there’s faecal contamination in your water—something that can cause serious illnesses like diarrhoea or cholera. According to the DWS’s 2023 Blue Drop Report, nearly half of SA’s water systems pose this kind of risk.
- Chemicals: This includes heavy metals (like lead), pesticides, and antiretroviral drugs (ARVs) such as lopinavir and efavirenz. These enter water sources when treatment plants can’t fully remove them, and some can be harmful if consumed over time.
- Physical Stuff (Look, Smell & Taste): Things like how cloudy your water is (turbidity), the pH level (acidity or alkalinity), and even its colour or smell can point to poor quality. These affect not just safety but whether water is even usable.
Tip: Want to know if your water is within safe limits? Look up the SANS 241:2015 standards on the Department of Water and Sanitation’s website.
Step 2: What You’ll Need to Test Your Water
Depending on how deep you want to go, there are different tools available:
- Home Testing Kits: Found online or hardware shops, kits from brands like Hach or LaMotte test for basics like pH, chlorine, hardness, and some bacteria. Expect to pay between R100 and R500.
- Sterile Containers: Grab these from a pharmacy or request them from a lab to collect clean, uncontaminated samples.
- Lab Testing (for more serious stuff like ARVs or heavy metals): If you want a thorough analysis, contact a lab like CSIR or WRC. They’ll test for things home kits can’t pick up—though it can cost between R500 and R2,000.
- Protective Gloves: To keep the sample clean, wear gloves when collecting your water.
Step 3: How to Collect a Water Sample the Right Way
Getting a clean sample is key to getting accurate results. Here’s how:
- Choose the Right Tap: Use the one you drink or cook from. If you suspect plumbing issues, collect the first water that comes out in the morning (it’s been sitting in the pipes).
- Sterilise the Tap: Clean it with an alcohol wipe or diluted bleach (1 part bleach to 10 parts water).
- Collect the Water: Fill a sterile container—use around 100–200 ml for bacteria tests or 500 ml–1 L for chemical testing. Don’t touch the inside of the bottle or the cap.
- Keep It Cold: Refrigerate your sample (around 4°C) and drop it off at the lab within 24 hours.
Step 4: Doing a Quick Test at Home
For general peace of mind, home kits are handy:
- Follow the Kit Instructions: Some use dip strips, others may need you to mix a solution or incubate samples for 24–48 hours.
- Check the Results: Match colour changes to the guide in the kit.
Here are a few reference points:
- E. coli: Should be completely absent in 100 ml of water.
- pH: Should sit between 6.5 and 8.5.
- Turbidity: Less than 5 NTU (basically how clear the water is).
Note: Home kits won’t pick up ARVs—you’ll need a lab test for that.
Step 5: Want Full Lab Results? Here’s What to Do
If you’re serious about knowing what’s in your water, especially ARVs or heavy metals, go pro:
- Find a Lab: Look for labs accredited by SANAS (or check with CSIR or WRC, as previously mentioned).
- Tell Them What to Test For: Ask for testing on bacteria (E. coli, coliforms), chemicals (like lead or ARVs), and general quality (pH, turbidity, etc.). ARVs are usually tested with special equipment called liquid chromatography-mass spectrometry.
- Get the Report: Labs will send you a breakdown of results and whether they meet national safety standards (SANS 241:2015).
Step 6: What to Do with the Results
Once you have your results:
- Compare to Standards: Even a tiny bit of E. coli or too much of a chemical (like lead above 10 µg/L) means your water isn’t safe.
- Take Action:
- Short Term: Boil your water for at least 1 minute or switch to bottled water that’s SABS-certified.
- Long Term: Install a proper water filter system (like reverse osmosis). WHO has guidelines for household filtration if you want to go deeper.
- Report It: If you find something worrying, notify your local municipality on the DWS complaints portal or email SAHRC at [email protected].
Step 7: Work Together as a Community
If your water is dodgy, odds are your neighbours’ water is too. Here’s how to tackle it together:
- Start a Water Committee: Club together to do batch testing—cheaper and louder as a group. SAWC has resources to help.
- Push for Accountability: Ask your municipality to show you their water quality reports (they’re legally required to provide these).
- Spread the Word: Work with local organisations like the SAWC or CER to get more eyes on the issue and more action from officials.
Step 8: Keep Checking–Don’t Let Up
Water quality can change depending on the season, rainfall, or pipe maintenance:
- Follow Official Reports: Check for updates in the Blue Drop, Green Drop, and No Drop reports from the DWS.
- Stay Updated with SAHRC: They regularly release water-related reports.
- Test Twice a Year: Once in the wet season (October–March) and again in the dry season (April–September). Contamination patterns can change with the weather.

By leveraging home testing kits, accredited laboratories, and official resources from the DWS, SAHRC, and WISA, residents can monitor water quality and push for enhanced treatment systems. Regular testing, combined with community-driven efforts and engagement with authorities, is essential to uphold the constitutional right to safe water and mitigate the risks posed by emerging contaminants.
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